| || || Narayan, Vinay Vikash.|
| || || Aerobic thermophilic bacteria from the Savusavu hot springs, Fiji Islands |
Author:Narayan, Vinay Vikash.
Institution: University of the South Pacific.
Call No.: pac In Process
Copyright:Under 10% of this thesis may be copied without the authors written permission
Abstract: Surveys of SavuSavu Hot Springs, Fiji’s largest and most active hot springs have been restricted to mostly geological descriptions. In the current investigation, a microbiological study was conducted to determine the presence of aerobic thermophilic bacteria. Samples were collected over four sampling periods between September 2005 and March 2006. 104 thermophilic bacterial isolates from these hot springs were characterized by staining, biochemical, molecular tests and the ability to produce extracellular hydrolytic enzymes. DNA was extracted using CTAB/chloroform-phenol double extraction method and subjected to 16S rDNA PCR with primers EUB A (R) and EUB B (F). Products were restriction digested using EcoRI and HaeIII, run on 2.5% TBE-Agarose gel and analyzed using UV AlphaImager. Majority of the isolates were Gram positive, produced endospores, and were motile. Catalase and oxidase activity were prominent and most isolates could utilize glucose in an oxidative manner. Amylase, lipase and gelatinase activities were also observed. Using Thermus and Bacillus strains as positive controls, 58% of the isolates were identified as Anoxybacillus flavithermus, 19% as Geobacillus stearothermophilus/Bacillus licheniformis, 10% as Thermus TG153 and 10% as Thermus TG206. Four of the isolates were unique in their molecular patterns suggesting that there may be novel bacteria in the Savusavu hot springs.