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close this section of the library Tuia, Valerie Saena.


View the PDF document In vitro multiplication of taro (Colocasia esculenta var. esculanta L. Schott)
Author:Tuia, Valerie Saena.
Institution: University of the South Pacific.
Award: M.Agri.
Subject: Taro -- Micropropagation, Taro -- Breeding , Taro -- Research -- Oceania
Date: 1997.
Call No.: pac SB 211 .T2 T84
BRN: 919788
Copyright:10-20% of this thesis may be copied without the authors written permission

Abstract: The study showed that in vitro multiplication rates of Colocasia esculenta var. esculenta vary from 5 to 38 suckers per plant per week due to the promoting effects of thidiazuron (TDZ), N-phenyl-N'-l-2-3-thiadiazol-5ylurea on sucker production. The use of different concentrations of TDZ indicated that the optimal concentrations of TDZ for shoot production in three out of five cultivars tested was 0.5 mg/l TDZ. The inclusion of 6- benzyl-aminopurine (BAP) in the Stage II medium had a significant effect on sucker development with optimal shoot regeneration at 0.8 mg/l BAP. The method which utilised both TDZ and BAP produced healthy and normal plantlels compared to those produced from treatments with TDZ only. When liquid medium was used at one or more of the different stages in the multiplication system, shoot proliferation and growth of cultures were improved. Culture time was also minimised with the use of liquid medium without affecting sucker numbers to a significant extent, but with increased time, further development of normal and fasciated suckers was encouraged. The effect of a chilling treatment (4 °C) on the development of shoots initiated by TDZ was evaluated. No significant effect was observed but the treatment could be useful as a short to medium term storage technique. A new tissue culture multiplication system developed based on results of this research is as follows: Stage 1 - Modified Murashige & Skoog Plant Salt Medium (1962) (MSO) + 0.5 mg/l TDZ for 4 weeks (solid medium), Stage II - MSO + 0.80 mg/l BAP for 6 weeks (solid medium), Stage III - MSO + 0.005 mg/l TDZ for 4 weeks (either in solid/liquid medium) and Stage IV - MSO for 4 weeks (in liquid medium).
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