| || || Polymerase chain reaction, Raw milk | Health aspects, Tuberculosis in cattle|
| || || Standardisation and application of Polymerase Chain Reaction (PCR) for determining the occurence of bovine tuberculosis in raw milk samples|
Author: Naaz, Zafiar Tasmeen
Institution: The University of the South Pacific
Subject: Polymerase chain reaction, Raw milk | Health aspects, Tuberculosis in cattle
Call No.: Pac QP 606 .D46 N33 2018
Copyright:20-40% of this thesis may be copied without the authors written permission
Abstract: Mycobacterium bovis occurs in a wide range of animals, especially farm animals of economic importance. It is emerging as one of the most important diseases that infect cattle. It also poses public health problem when transmitted to humans by either inhaling infected aerosols or ingesting M. bovis through contaminated meat and milk products. In the current study, a PCR method was standardised to use for the detection of bovine tuberculosis in Fiji. No previous work has addressed detection of M. bovis using PCR in Fiji, so various steps of optimisation were involved, in which a positive control was mandatory. Therefore, a positive control was first obtained from the Ministry of Agriculture (MoA) that isolates M. bovis bacteria in the veterinary lab using organs showing lesions during post mortem surveillance.